Calculating Similarities: Normalizing Values
Next you normalize the values by subtracting the mean for that gene and then dividing it by the standard deviation.
For example, Gene A–Sample 1 becomes:
(0.602 − 0.305) ÷ 0.254 = 1.167
| Gene A | Gene B | Gene C | Gene D | |
|---|---|---|---|---|
| Sample 1 | 1.166744 | 0.099756 | −0.53475 | −0.49276 |
| Sample 2 | −0.01659 | −0.39902 | 1.074453 | 0.075694 |
| Sample 3 | 0.938726 | 1.649106 | −0.93956 | 1.885779 |
| Sample 4 | −0.50801 | −1.4496 | −0.93956 | −0.49276 |
| Sample 5 | −1.58087 | 0.099756 | 1.339419 | −0.97595 |